HAYATOGLU
Your scientific support
Examples of tests that are conducted:
OUR STUDIES
One approach to the development of anticancer drugs is known to be action on the metabolism of cancer cells. The main targets used are pyruvate kinase (PK) in glycolysis, glutaminase in the glutaminolysis pathway and isocitrate dehydrogenase (IDH) in the tricarboxylic acid cycle (TCA).
The main targets used are pyruvate kinase (PK) in glycolysis, glutaminase in the glutaminolysis pathway and isocitrate dehydrogenase (IDH) in the tricarboxylic acid cycle (TCA).
There is also evidence that mitochondrial metabolism is active and necessary for tumourgrowth. Mitochondrial metabolism supports tumour anabolism by providing key metabolites for macromolecule synthesis and generating oncometabolites to maintain the cancerous phenotype.
Our team has the ability to carry out the above tests and would also be happy to take on any new experiment.
Cell Metabolism Testing
The study of drug-protein binding can be divided into two main groups: separation methods (which allow the calculation of binding parameters, i.e. the number of binding sites and their corresponding affinity constants) and non-separation methods (which describe mainly qualitative parameters of the ligand-protein complex).
We follow the latest trends and also take into account stereoselective and non-stereoselective aspects using chromatography, capillary electrophoresis and microdialysis.
With us you get a detailed picture of the protein-protein interaction of your compounds. This is essential for further research and optimisation of the lead compound.
Of course, we also carry out Flow Cytometry Testing, Two Dimensional Gel Electrophoresis, Western Blot Testing.
Protein Binding Studies
In addition to the obvious application of the test - assessing the decrease in cancer cell growth in the presence of the molecules under investigation - this test can also be used in immunotoxicitytests.
These are important in determining the toxic effects of various chemicals on the immune system. The immune system is a direct target for numerous toxicants that have health consequences such as susceptibility to infections, cancer, allergic reactions and autoimmune diseases.
One way to investigate the harmful effects of various chemicals is to study apoptosis and/or proliferation in immune cells. However, when affected by a toxicant, increased apoptosis of immune cells can cause immunosuppression, leading to the development of cancer and an inability to fight infections.